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miR-7a2 is expressed in the endocrine lineage of developing mouse pancreas (A) RT-qPCR of miR-7a1 and miR-7a2 (miR-7a), miR-7b and Neurog3 in mouse pancreatic buds at e12.5–18.5 and P3. Data are mean ± SEM. n = number of embryos/pups, n = 4 for e12.5–14.5 and P3, n = 7 for e15.5–18.5, (B and C) Representative image of a miR-7a2 LacZ/LacZ mouse pancreatic bud at (B) e14.5 and (C) e18.5, stained with DAPI (blue), or with antibodies against E-cad (green), β-gal (red) and Muc1 (white), (D and E) Representative image of a miR-7a2 LacZ/LacZ mouse pancreatic bud section at e18.5, stained with DAPI (blue), and with antibodies against β-gal (red) and (D) insulin (Ins) (green) and glucagon (Gcg) (white), or (E) somatostatin (Sst) (green). LacZ gene product β-gal reports endogenous miR-7a2 expression. Scale bar = 50 μm.

Journal: iScience

Article Title: MicroRNA-7 regulates endocrine progenitor delamination and endocrine cell mass in developing pancreatic islets

doi: 10.1016/j.isci.2024.110332

Figure Lengend Snippet: miR-7a2 is expressed in the endocrine lineage of developing mouse pancreas (A) RT-qPCR of miR-7a1 and miR-7a2 (miR-7a), miR-7b and Neurog3 in mouse pancreatic buds at e12.5–18.5 and P3. Data are mean ± SEM. n = number of embryos/pups, n = 4 for e12.5–14.5 and P3, n = 7 for e15.5–18.5, (B and C) Representative image of a miR-7a2 LacZ/LacZ mouse pancreatic bud at (B) e14.5 and (C) e18.5, stained with DAPI (blue), or with antibodies against E-cad (green), β-gal (red) and Muc1 (white), (D and E) Representative image of a miR-7a2 LacZ/LacZ mouse pancreatic bud section at e18.5, stained with DAPI (blue), and with antibodies against β-gal (red) and (D) insulin (Ins) (green) and glucagon (Gcg) (white), or (E) somatostatin (Sst) (green). LacZ gene product β-gal reports endogenous miR-7a2 expression. Scale bar = 50 μm.

Article Snippet: Chicken anti-β-galactosidase (1:100) , Abcam , Cat # AB9361; RRID: AB_307210.

Techniques: Quantitative RT-PCR, Staining, Expressing